How it works
Why our kits do not need a freezer
Most reagents are formulated for a lab at the end of an unbroken cold chain. We dry ours into a glass instead, so heat stops being a risk we manage and becomes something we designed around.
The process
Four steps from enzyme to sealed dose
Every product uses the same process, which is why a new kit gets the room-temperature shelf life without starting over.
01
Pick an enzyme that survives drying
We screen thermostable polymerase variants for how much activity they keep after being dried out. Most of the work is choosing the right starting enzyme, not rescuing a bad one later.
02
Set it in a glass
A trehalose–mannitol scaffold vitrifies around the enzyme. In a glass, molecules cannot move, and an enzyme that cannot move cannot unfold.
03
Freeze-dry it
Controlled primary and secondary drying pull residual moisture below 2%. That is the line above which the glass stops behaving like a glass in tropical heat.
04
Seal it under nitrogen
Foil-sealed single doses that need nothing but water to come back. No freezer, no gel pack, no courier chain to break.
Stability
How much yield survives
Measured against a 4 °C control lot
We hold each formulation under real-time and accelerated conditions, then run it against a refrigerated control. What matters is not whether the enzyme is still there. It is whether the reaction still gives you the band.
- Residual moisture
- < 2%
- Validated cycles
- 35 per formulation
- Validated amplicon
- 5 kb
- To reconstitute
- Water only
Yield kept, by storage condition
4 °C · Reference
100%
25 °C · 12 months
98%
37 °C · 6 months
96%
45 °C · 4 weeks
94%
Stability is measured under tropical conditions rather than extrapolated from a temperate model, because that is where these kits have to survive.
How we make it
Formulation first, volume second
The chemistry is done and the drying cycles are validated. Production capacity is what we are building now, which is why we would rather put kits in a few labs and be corrected than scale something nobody has stress-tested.
Quality control
Four checks before it reaches you
01
Incoming materials
Identity, endotoxin and nuclease screening on every raw material lot.
02
In process
Fill weight, moisture and vacuum integrity checked on every lyophilizer shelf.
03
Release
PCR yield, sensitivity and a no-template control run on each batch.
04
Stability
Real-time and accelerated stability, tracked continuously as the shelf life claim is earned.
The cycle
Freeze, dry, seal
The reagent is frozen solid, then the ice is pulled off as vapour under vacuum rather than melted. What is left is a dry cake with the enzyme suspended in it, sealed before it can pick up moisture again.
Add water at the bench and the whole thing comes back in seconds.
Four weeks at 45 °C
And it still gives you the band